CELL CULTURE

Isolation and Culture of Primary Cell Lines

Cytonex Laboratory is certified as Cleanroom – Grade C compliant by the National Environmental Balancing Bureau. The lab is well equipped with Class II biosafety cabinets, CO2 incubators, centrifuges, automated cell counter and inverted microscope which are essential for cell culture work. Our company is driven by a young, motivated, experienced and professional team to perform isolation and culture work. We provide various cell lines, such as:

EU GMP Standard Cleanroom ​

Our cells are cultured in an ISO9001:2015 certified & EU GMP standard cleanroom.

Cryopreservation ​

Cells are kept in cryo-storage tank at ultra-low temperature ( -196 °C).

Quality Assurance (Morphology) ​

Each batch of our cells are checked for morphology using inverted microscope to monitor cell growth.
Flow cytometer
Multiple characteristics of a single cell can be studied using flow cytometer when the cells are stained with specific antibodies conjugated with fluorochromes.

Flow Cytometry

Services such as further characterization of stem cells or other cells by detecting and measuring the physical and chemical characteristics of the population of cells. High quality equipment, BD FACSVia flow cytometer, equipped with blue (488 nm) and red laser (640 nm) to support a wide range of studies:

  • Immunophenotyping
  • Mitochondria membrane potential study using Tetramethylrhodamine, ethyl ester (TMRE)
  • Cell Cycle analysis
  • Cell viability analysis using 7-Amino-Actinomycin D (7-AAD)
  • Any other suitable kits
Epoch Microplate Spectrophotometer
Epoch microplate spectrophotometer controlled with the Gen5 Software interface provides precise UV/Vis measurements for different applications.

Microplate Based

The Epoch Microplate Spectrophotometer offers a wide wavelength range (200 nm to 999 nm) for UV-Vis absorbance measurements to support the following tests:

  • XTT assay
  • ELISA