Extracellular vesicles (EVs) isolation, quantification and characterization
Extracellular vesicles (EVs) have gained attention in regenerative
medicine due to their unique characteristics. Natural
EVs are membraneous vesicles released by cells into the
extracellular space that play a role in cell-to-cell communication.
Through this system, cells are capable of exchanging
DNA, RNA, proteins, and lipids to neighboring and
remote sites in the body. EVs are categorized into different
groups such as exosomes, microparticles, and apoptotic
bodies. There are multiple different methods have been developed
to isolate, quantify and characterize EVs. Once isolated,
EVs can be quantified and characterized.
Isolation
Ultracentrifugation is the most commonly used primary
extracellular vesicles (EVs) separation and
concentration technique to isolate mesenchymal
stem cell derived EVs. We isolate mesenchymal
stem cell derived EVs with our optimized isolation
method by using our latest ultracentrifuge, Beckman
Coulter Optima XE-100 Ultracentrifuge.
Ultracentrifuge
Beckman Coulter
Passage
MSCs
MSCs
Unisolated
EVs
EVs
Ultra
centrifugation
centrifugation
Isolated
EVs
EVs
Extracellular Vesicles Immunophenotyping (Flow cytometer)
We study our extracellular vesicles (EVs) specific
surface markers by using our latest high end flow
cytometer, the BD FACSCanto II Flow Cytometry
Systems that can deliver reliable accuracy for our
EVs study. With BD FACSCanto II, EVs can undergoes
stringent surface markers detection test, to
determine the quality and purity to ensure desired
standards are met.
Isolated
EVs
EVs
Flow Cytometry
Analysis
Analysis
Sample EVs
Analysis
Analysis
Real Time Extracellular Vesicles Size Determination Analysis
Nanoparticle Tracking Analysis (NTA) is one of the
method to characterize extracellular vesicles (EVs)
in real time. Nanoparticle Tracking Analysis (NTA)
allows specific EVs and microvesicles in the range
of 50-1000 nm in liquid suspension to be directly
and individually visualized and counted in real-time.
Simultaneously, NTA provides high-resolution
particle size distribution profiles and concentration
Malvern Panalytical
Nanosight NS300
Isolated
EVs
EVs
Nanoparticle
Tracking Analysis
Tracking Analysis
Sample
NTA Analysis
NTA Analysis
Why Cytonex
Cytonex has extensive experience in extracellular vesicles (EVs) exploration including isolation,
quantification and characterization. Utilizing our expertise in EVs field, Cytonex offer varities EVs
laboratory services including EVs isolation, immunophenotyping (flow cytometer), and real-time
EVs size determination analysis to help you better define your EVs and facilitate the
downstream applications.
