Extracellular vesicles (EVs) isolation, quantification and characterization

Extracellular vesicles (EVs) have gained attention in regenerative medicine due to their unique characteristics. Natural EVs are membraneous vesicles released by cells into the extracellular space that play a role in cell-to-cell communication. Through this system, cells are capable of exchanging DNA, RNA, proteins, and lipids to neighboring and remote sites in the body. EVs are categorized into different groups such as exosomes, microparticles, and apoptotic bodies. There are multiple different methods have been developed to isolate, quantify and characterize EVs. Once isolated, EVs can be quantified and characterized.

Isolation

Ultracentrifugation is the most commonly used primary extracellular vesicles (EVs) separation and concentration technique to isolate mesenchymal stem cell derived EVs. We isolate mesenchymal stem cell derived EVs with our optimized isolation method by using our latest ultracentrifuge, Beckman Coulter Optima XE-100 Ultracentrifuge.

Ultracentrifuge
Beckman Coulter

Passage
MSCs
Unisolated
EVs
Ultra
centrifugation
Isolated
EVs

Real Time Extracellular Vesicles Size Determination Analysis

Nanoparticle Tracking Analysis (NTA) is one of the method to characterize extracellular vesicles (EVs) in real time. Nanoparticle Tracking Analysis (NTA) allows specific EVs and microvesicles in the range of 50-1000 nm in liquid suspension to be directly and individually visualized and counted in real-time. Simultaneously, NTA provides high-resolution particle size distribution profiles and concentration

Malvern Panalytical
Nanosight NS300

Isolated
EVs
Nanoparticle
Tracking Analysis
Sample
NTA Analysis

Why Cytonex

Cytonex has extensive experience in extracellular vesicles (EVs) exploration including isolation, quantification and characterization. Utilizing our expertise in EVs field, Cytonex offer varities EVs laboratory services including EVs isolation, immunophenotyping (flow cytometer), and real-time EVs size determination analysis to help you better define your EVs and facilitate the downstream applications.

Let’s discuss your testing requirements